Abstract
A high molecular weight (HMW) protein with apparent MW over 450 kilodaltons was isolated from rat kidney proximal tubular brush border (BB) membrane fraction by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis. Rabbit antibodies raised against this component were specific for the HMW protein, as shown by the immunoblotting assay. The antibodies reacted with the BB area of the proximal tubules both in rat and human kidneys, and gave a faint reaction also with the capillary walls of glomerules, as seen after immunofluorescence staining of frozen sections. This antiserum and a rabbit antiserum made against the whole BB membrane fraction were used to study the expression of proximal tubular antigens in 30 human renal carcinomas. 80 % of the carcinomas were positive for BB antigens and of these 75 % expressed the HMW protein. In poorly differentiated carcinomas the HMW antigen had a diffuse, cytoplasmic distribution, while in the carcinomas forming tubulus-like structures the antigen was polarized at the apical and basement membrane regions of the cells.
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