Abstract
Abstract Objectives The aim of this study was to investigate BAP1, OGT and YY1 genes and protein levels in 12 samples (8 males, 4 females) of eyelid tumor tissue with basal cell carcinoma (BCC) and 12 normal control subjects (8 males, 4 females). Methods The expression levels of these genes were determined with RT-PCR and the protein levels and expression using ELISA and IHC methods, respectively. Results In RT-PCR analysis, statistically significant upregulated expression was determined of 1.84-fold of BAP1, 2.85-fold of OGT and 3.06-fold of YY1 genes (p < 0.05). In the patient group, compared to the control group, there was a similar statistically significant strong correlation between the proteins (BAP1 and YY1; r = 0.850, BAP1 and OGT; r = 0.811, OGT and YY1; r = 0.755) (p < 0.05). In the ELISA and IHC analysis methods, a significant increase in BAP1 and YY1 protein expression levels was observed compared to the control group (p < 0.05). Conclusions The study results demonstrated that BAP1 and YY1 genes and protein levels were upregulated in eyelid tumor tissue with BCC.
Highlights
Benign or malignant lesions may be observed around the eyelid [1]
The study results demonstrated that BAP1 and Yin Yang 1 (YY1) genes and protein levels were upregulated in eyelid tumor tissue with basal cell carcinoma (BCC)
Malignant eyelid tumors are rarely observed, but the diagnosis and treatment of such tumors are the focus of interest of eye specialists [2]
Summary
Benign or malignant lesions may be observed around the eyelid [1]. Malignant eyelid tumors are rarely observed, but the diagnosis and treatment of such tumors are the focus of interest of eye specialists [2]. The risk of eye melanoma has been reported to increase in some people with mutations in the BAP1 tumor suppressor gene [5]. Recent studies have shown that mutations in the BAP1 gene are associated with various types of cancer [9]. Protein glycolysis catalyzed by the OGT enzyme is a post-translational modification by O-linked N-acetylglycoamination (O-GlcNAc). The clinical significance of changes in the O-GlcNAc signal and OGT abnormal expression in cancer is not yet fully known. Some studies of cancer patients have shown the overexpression of both OGT and O-GlcNAc modifications in cancer tissues [15]. The aim of the current study was to determine the expression levels of BAP1, OGT, and YY1 genes and proteins in eyelid tumor tissue with BCC
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