Abstract

ObjectiveTo investigate the expression and significance of telomerase activity and telomerase reverse transcriptase(TERT)gene in hRPE cells.MethodsUsing in situ hybridization(ISH)and telomerase repeat amplification protocol(TRAP),the expression of hTERT gene and telomerase activity in hRPE cells was detected.ResultsThe hTERT gene was expressed in hRPE cells with in situ hybridization technique.Low levels of telomerase activity were detected with TRAP.ConclusionsThe results suggest that detection of telomerase hTERT gene and telomerase activity is valuable to PVR genetic medication. Key words: human retinal pigment epithelium cells telomerase activity hTERT gene in situ hybridization telomeric repeat amplification protocol

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