Expression and significance of miRNA-126 in patients with coronary in-stent restenosis

Abstract

Objective To observe the differences of miR-126 expression in peripheral blood of patients with coronary in-stent restenosis (ISR) and evaluate its significance. Methods Twenty-six cases of patients with ISR in the Cardiology of Affiliated Hospital of Jining Medical University from June 2014 to December 2015 were collected as ISR group, 40 patients without ISR as non-ISR group.The clinical data and coronary stenting data were calculated.The differences of miR-126 expression levels which were detected by real-time PCR were compared by t test between the two groups.The ROC curve was used to analyze the predicted value of miR-126 on ISR after coronary stenting.Bioinformatics predicted the target genes of miR-126 and performed functional analysis and enrichment of these target genes.The main biological functions of miR-126 and its possible mechanism in ISR were discussed. Results Comparison between the ISR and non-ISR group in terms of coronary lesion characteristics and surgical characteristics: there were no significant differences in the diameter of the stent [(3.21±0.88)mm, (3.15±0.67)mm], the length of the stent [(20.83±4.92)mm, (21.02±4.75)mm] and the maximum pressure of the stent release [(14.17±9.1)atm, (13.97±6.26)atm] (t=0.97, -1.04, 0.87; P>0.05). The expression of miR-126 in ISR group (0.63±0.38) was lower than that in non-ISR (1.39±1.03), the difference was statistically significant (t=-5.46, P<0.01). AUC of baseline miR-126 on the coronary stent after 12-18 months of ISR was 0.791 (95% CI 0.671 ~ 0.912, P<0.01). The targets gene of miR-126 were predicted by the database, and the function of these target genes was enriched.Its main directions were vascular endothelial growth factor signaling pathway, extracellular matrix and cell proliferation regulation. Conclusions miR-126 is associated with an increased risk of ISR during postoperative follow-up, and it is a predictor of ISR in patients with coronary stent.The biological function of miR-126 is mainly focused on the direction of vascular endothelial growth factor signaling pathway, which may be a way to participate in the ISR process. Key words: Coronary artery disease; In-stent restenosis; MicroRNAs; Computational biology