Expression and Significance of Matrix Metalloproteinase-9, Cyclooxygenase-2 mRNA in Patients With Premature Rupture of Membranes

Abstract

Objective To investigate the expression and its significance of human metalloproteinase-9, cyclooxygenase-2 in membrane of premature rupture of membranes (PROM) and normal term pregnancy. Methods We selected 35 subjects from the pregnant women of PROM in our hospital from September 2011 to February 2012 as research group. All subjects were divided into term PROM group (tPROM group, n=10) and preterm PROM group (pPROM group, n=25). The average age of tPROM group was (29.71±4.63) years old, and the average gestational age was (38.04±0.79) weeks. The average age of pPROM group was (30.67±4.05) years old, and the average gestational age was (33.56±0.82) weeks. Meanwhile, we selected 25 subjects from the healthy term-pregnant women as control group. The average age was (30.46±4.14) years old, the average gestational age was (38.91±0.99) weeks. This study followed the procedures established by the committee meet human trials hospital ethical standards, obtained its approval. All grouping subjects were informed and consent, and signed clinical research informed consent. The messenger RNA(mRNA) of MMP-9, COX-2 in fetal membranes by using real-time PCR in different groups were examined and compared. Results ①The mRNA expressions of MMP-9 and COX-2 in fetal membranes were significantly high in pPROM group and tPROM group than those in control group (P 0.05). ②The relationship between MMP-9 and COX-2 in pPROM group and tPROM group was positively correlated (r=0.732, 0.820, P 0.05). Conclusions The mRNA expressions of MMP-9 and COX-2 in fetal membranes were significantly high in PROM, and they may play key roles in the occurrence of PROM. Key words: premature rupture of membranes; matrix metalloproteinase-9; cyclooxygenase-2; real-time PCR