Abstract
Objective To explore the potential changes of connexin Cx36 in hippocampus and cortical neurons of rats with hyperthermia-induced convulsion. Methods Rats were divided into 2 groups according to the random number table method: normal control group and experimental group.Febrile convulsion model was elicited through immersion in warm water.The experimental group was generated following febrile convulsion model: hyperthermia group and febrile convulsion group.Among normal control group, hyperthermia group and febrile convulsion group, western blot analysis and immunofluorescence labeling techniques were used to examine the expression of Cx36 protein in the hippocampus and cortex area.One-Way ANOVA was used to compare the mean of multiple sample, the LSD test was used to compare the two means. Tamhane′s test was used when variance were uneven. Results The incubation period, seizure duration and temperature were (4.39±0.08) min, (5.38±0.07) min, (41.87±0.06) ℃ after hyperthermia-induced convulsion, respectively. Western blot analysis showed that the expression of Cx36 protein in the hippocampus and cortex area decreased gradually after 10 times of seizure in normal control group, hyperthermia group and febrile convulsion group, and the febrile convulsion group decreased most obviously.Compared with normal control group and hyperthermia group, respectively, in febrile convulsion group Cx36 expression obviously decreased in the hippocampus and cortex in rats with 1, 5, 10 seizure times induced by hyperthermia, and with the increase of number of induced convulsion, the expression of Cx36 was significantly decreased in the cortex (0.104± 0.012) and CA1 (0.091±0.011), CA3 (0.090±0.011) and DG (0.092±0.012) areas of hippocampal neurons compared with the normal control group (0.212±0.017, 0.167±0.013, 0.159±0.014, 0.171±0.013) and the hyperthermia group (0.189±0.006, 0.144±0.008, 0.129±0.005, 0.165±0.011) (all P<0.05). Furthermore, the extent of reduction in Cx36 expression seemed to correlate with the number of seizures. Conclusion With the increase of thermal seizure frequency, Cx36 expression of rats was decreased obviously which may lower convulsion threshold and lead to recurrent seizures. Key words: Febrile seizure; Cx36; Rat; Hippocampus; Cortical neurons
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