Abstract
Objective To investigate the expression of caudal-related homeobox transcription factor 2 (CDX2) and its role in placenta of patients with preeclampsia (PE). Methods Thirty cases of PE women who delivered in our hospital from September 2015 to April 2016 were recruited. Thirty cases of the first trimester and 30 cases of full-term birth women at the same period were also recruited. Reverse transcription (RT)-PCR was used to detect the expression of CDX2 mRNA in placenta. Western blotting was used to detect the expression of CDX2 protein in placenta. Immunohistochemistry SP method was applied to detect the expression and the location of CDX2 in placenta. Results ①RT-PCR showed that the CDX2 mRNA expression was significantly lower in PE (0.385±0.065) than that in the first trimester (1.880±0.235) and full-term birth (1.015±0.184) women groups. There was a significant difference (P<0.05). ②Western blotting showed that the CDX2 protein expression was significantly lower in the placenta of PE (0.276±0.029) than in the first trimester (0.658±0.059) and full-term birth women (0.478±0.136) (P<0.05). ③Immunohistochemical detection showed that the CDX2 protein was expressed in the placentas of full-term birth women and PE women of the first trimester. The expression of CDX2 in placenta mainly distributed in the cytoplasm of placental syncytiotrophoblasts. The expression of CDX2 was significantly decreased in PE group. Conclusion The down-regulation of CDX2 may be involved in the pathogenesis of PE, suggesting that it may be involved in trophoblast proliferation, invasion and placental formation. Key words: Caudal-related homeobox transcription factor 2 (CDX2); Placenta; Trophoblast; Preeclampsia (PE)
Published Version
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