Expression and its significance of microRNA-210 in serum in acute cerebral infarction

Abstract

To investigate the expression of serum microRNA-210 (miR-210) in patients with acute cerebral infarction (ACI), and to evaluate its clinical significance. A retrospective study was conducted. Eighty patients with ACI admitted to Tianjin Hospital from January 2011 to March 2014 within 48 hours of onset were enrolled, and 30 healthy volunteers served as controls. The peripheral blood was collected, and the expression of serum miR-210 was determined by reverse transcription-polymerase chain reaction (RT-PCR). The receiver operating characteristic curve(ROC) was drawn to analyze the role of miR-210 in the diagnosis of ACI. According to the pathological and physiological characteristics of patients receiving treatment, the relationship between miR-210 and clinical physiology index was analyzed. The expression of miR-210 in serum of patients with ACI was significantly lower than that of the healthy control group (2(-Δ ΔCt): 1.349 ± 0.043 vs. 1.923 ± 0.107, t=6.567, P<0.000). ROC analysis results showed that the sensitivity of miR-210 in the diagnosis of ACI was 90.4%, the specificity was 76.2%, and the area under the ROC (AUC) was 0.804 [95% confidence interval (95%CI)=0.700-0.908]. No difference in expression of miR-210 (2(-Δ ΔCt)) in serum was found in patients of different gender, age, and infarction area (male and female: 1.33 ± 0.13 and 1.31 ± 0.06,t=3.562, P=0.473; ≤ 60 years and >60 years: 1.32 ± 0.12 and 1.31 ± 0.09, t=2.351, P=0.264; large infarction, small infarction, lacunar infarction: respectively 1.31 ± 0.02, 1.33 ± 0.11, 1.31 ± 0.06, F=1.236, P=0.087), or with the severity of cerebral infarction, and there was a tendency in lowering of expression of miR-210 (2(-Δ ΔCt), light, medium, severe: 1.53 ± 0.11, 1.33 ± 0.11, 1.08 ± 0.04, F=5.394, P=0.014). The serum level of miR-210 in ACI was significantly lower than that in normal healthy persons, and it may be an important new serological marker in screening and diagnosis of ACI.