Abstract
This study aimed to investigate the expression and function of ryanodine receptors (RyRs) in rabbit penile corpus cavernosum smooth muscle (CCSM) cells. New Zealand white rabbit CCSM cells were cultured by primary tissue culture and identified by immunofluorescence technique. mRNA of three RyRs subunits in cultured CCSM cells was detected by reverse transcription polymerase chain reaction (RT-PCR). After excitation by noradrenaline, the concentration of Ca(2+) in CCSM cells was detected by laser scanning confocal microscopy. It was found that only the RyRs1 subunit is expressed in CCSM by RT-PCR. The CCSM cells were divided into two groups: Group A, CCSM cells + 10 micromol l(-1) noradrenaline and Group B, CCSM cells + 50 micromol l(-1) procaine 10 micromol l(-1) noradrenaline. Compared with the base level and the level after noradrenaline excitation, fluorescence intensity improved 44.10 +/- 6.01% in the test group A (n = 8) and 32.92 +/- 4.92% (n = 8) in the procaine control group B, respectively. There were statistically significant differences between group A and group B (P < 0.01). It is concluded that RyRs1 subunit is expressed in CCSM cells and may contribute to regulating the cytoplasmic Ca(2+) level.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
