Expression and clinical significance of combined detection of Cullin1 and MT4-MMP in breast cancer

Abstract

Objective To explore the expression and clinical significance of Cullin1 and MT4-MMP in different types of breast cancer tissue. Methods Eighty cases of breast cancer tissues and 80 cases of normal breast tissues adjacent to breast cancer were selected. Immunohistochemistry and Western blotting was used to detect the expression of Cullin1 and MT4-MMP in cancer tissue and normal breast tissue respectively between January 2015 and August 2015 in Xuzhou City Cancer Hospital, and to analyze the correlation between Cullin1 and MT4-MMP. Results The expressions of Cullin1 and MT4-MMP in breast cancer were significantly higher than in adjacent normal breast tissues (77.5% vs 28.8%, 67.5% vs 17.5%, χ2=38.174, 40.921, both P<0.01). In breast cancer tissues, the expression of Cullin1 and MT4-MMP protein was positively correlated (P<0.05). The expression of Cullin1 and MT4-MMP in breast cancer was statistically significant in molecular typing, axillary lymph node metastasis and TNM staging (P<0.05), but without significant difference in the age, tumor size, tumor location, recurrence and metastasis. Conclusions Cullin1 and MT4-MMP may be involved in the formation and development of breast cancer and seem to play a certain synergy role in this process. Higher expression level of Cullin1 and MT4-MMP in worse molecular typing and advanced breast cancer may become a new marker of malignant breast cancer. Key words: Breast neoplasms; SKP Cullin F-box protein ligases; Matrix metalloproteinase 17