Abstract
The Chinese citrus fly, Bactrocera minax, is a devastating pest of citrus, which enters the obligatory diapause in overwintering pupae to resist harsh environmental conditions. However, little is known about the molecular mechanisms underlying pupal diapause. The previous transcriptomic analysis revealed that a large number of genes were regulated throughout the pupal stage. Of these genes, 12 and six ones that are remarkably up- and downregulated, respectively, specifically in intense diapause were manually screened out in present study. To validate the expression of these genes throughout the pupal stage, the quantitative real-time PCR (qRT-PCR) was conducted, and the genes displaying different expression patterns with those of previous study were excluded. Then, the expressions of remaining genes were compared between diapause-destined and non-diapause-destined pupae to reveal their association with diapause using qRT-PCR and semiquantitative PCR. Finally, five genes, TTLL3B, Cyp6a9, MSTA, Fru, and UC2, and two genes, KSPI and LYZ1, were demonstrated to be positively and negatively associated with diapause, respectively. These findings provide a solid foundation for the further investigation of molecular mechanisms underlying B. minax pupal diapause.
Highlights
The Chinese citrus fly, Bactrocera minax (Enderlein) (Diptera: Tephritidae), an oligophagous pest of citrus fruits, has been considered as a devastating pest of citrus plants in the temperate areas ofAsia [1,2]
These findings provide a solid foundation for the further investigation of molecular mechanisms underlying B. minax pupal diapause
According to our previous transcriptomic study, over 4000 genes were regulated throughout the the pupal stage of B. minax, and several physiological pathways were deployed in diapause pupal stage of B. minax, and several physiological pathways were deployed in diapause programming [17]
Summary
The Chinese citrus fly, Bactrocera minax (Enderlein) (Diptera: Tephritidae), an oligophagous pest of citrus fruits, has been considered as a devastating pest of citrus plants in the temperate areas ofAsia [1,2]. The outbreaks of B. minax in several provinces of China have caused serious economic losses to the citrus industry [2,3]. This pest has aroused great concerns in citrus-growing regions in China and the research on this pest has been widely conducted [4,5,6,7,8,9,10,11]. The univoltine B. minax enters the pupal diapause to overwinter. The overwintering pupal stage lasts for 160–170 d, synchronizing the adult emergence with citrus fruit bearing [15]. Understanding the molecular mechanisms of pupal diapause of B. minax will contribute to elucidating the inherent mechanisms underlying
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