Abstract
Abstract: This comprehensive review paper focuses on LC-ESI-MS/MS, a powerful analytical technique widely used in various scientific disciplines. It begins by explaining the working mechanisms and components of LC-ESI-MS/MS highlighting advancements in sensitivity, resolution, speed and data analysis. This paper explores the diverse applications of LC-ESI-MS/MS in pharmaceutical analysis, environmental analysis, metabolomics, and proteomics, discussing its advantages and limitations in each area. The review also emphasizes the integration of LC-ESI-MS/MS with other techniques like GC-MS, CE-MS, and MALDI-MS, showcasing their combined effects and enhanced capabilities. It presents recent developments and innovations in LC-ESI-MS/MS technology including improvements in sensitivity, resolution and data analysis. This paper discusses emerging trends in sample preparation, ionization methods, chromatographic columns, and mass spectrometry detectors. Addressing the challenges and limitations, the review identifies potential areas for improvement and future research directions. It emphasizes the untapped potential of LC-ESI-MS/MS in advancing scientific knowledge and solving complex analytical problems, calling for further collaboration and research in the field. In conclusion, this review paper provides a comprehensive overview of LCESI-MS/MS, emphasizing its fundamental principles, applications, recent innovations, and future perspectives. The findings underscore the significance of LC-ESI-MS/MS in modern science and its potential for further advancements.
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