Exploring the Sequence analysis of the Two-Component System Response Regulator OmpR in Multi-Drug Resistant (MDR) Acinetobacter baumannii

Abstract

Background: The study focuses on the clinical profiles, antibiotic susceptibility, and genetic characteristics of 25 A. baumannii isolates from patients with urinary tract infections (UTIs). Patient Cohort: Ages range from 18 to 70 years, with a male to female ratio of 1.375. Notable Finding: Some multidrug-resistant (MDR) isolates had commonalities with extensively drug-resistant (XDR) variations, showing the evolution of drug resistance.Methods: Antibiotic Efficacy Analysis: Evaluation of antibiotic efficacy when the variable moved from lower (Bin 1) to higher (Bin 2) levels. Correlation Matrix: An analysis of antibiotic correlations to better identify potential cross-resistance and shared characteristics. Genetic Diversity Analysis: An examination of variants in the OmpR gene, including mutations and polymorphisms. Sequence analysis is used to identify point mutations in OmpR, with an emphasis on transitions such as adenine (A) to guanine (G).Results: Significant improvement in antibiotic efficacy from Bin 1 to Bin 2. Correlation Findings: Antibiotics have complex interactions, which may indicate cross-resistance. Genetic diversity: Variations in the OmpR gene have implications for virulence and adaptability. Sequence Analysis: The majority of point mutations in OmpR were transitions, with A typically changing to G.Conclusion: In Iraq, researchers have discovered the first evidence that clinical resistance in A. baumannii may be caused by structural alterations in the OmpR gene. A. baumannii isolates' genetic diversity at certain locations suggests possible implications on virulence and adaptation.Keywords: Acinetobacter baumannii; OmpR; Polymorphism; MDR