Abstract
The Corneal wound healing results in the formation of opaque corneal scar. In fact, millions of people around the world suffer from corneal scars, leading to loss of vision. This study aimed to identify the key changes of gene expression in the formation of opaque corneal scar and provided potential biomarker candidates for clinical treatment and drug target discovery. We downloaded Gene expression dataset GSE6676 from NCBI-GEO, and analyzed the Differentially Expressed Genes (DEGs), Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analyses, and protein-protein interaction (PPI) network. A total of 1377 differentially expressed genes were identified and the result of Functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) identification and protein-protein interaction (PPI) networks were performed. In total, 7 hub genes IL6 (interleukin-6), MMP9 (matrix metallopeptidase 9), CXCL10 (C-X-C motif chemokine ligand 10), MAPK8 (mitogen-activated protein kinase 8), TLR4 (toll-like receptor 4), HGF (hepatocyte growth factor), EDN1 (endothelin 1) were selected. In conclusion, the DEGS, Hub genes and signal pathways identified in this study can help us understand the molecular mechanism of corneal scar formation and provide candidate targets for the diagnosis and treatment of corneal scar.
Highlights
In the present study, we downloaded an original microarray data set GSE6676 from the NCBI Gene Expression Comprehensive Database (NCBI-GEO) and analyzed to obtain Di erentially Expressed Genes (DEGs) between mice that have the overexpression of TGF beta and wildtype litter mate to the mice that have the overexpression of TGF beta
There are no researches about di erential gene analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analyses, and protein-protein interaction (PPI) network analyses of corneal scar using the dataset of GEO database. erefore, gene expression pro le analysis can better understand the genetic, cellular, and molecular changes that occur during the formation of corneal scar, which will provide an opportunity to design treatment options, selectively regulate the key stages of healing process, and produce scars closer to normal corneal structure [5]
Scar formation of the cornea is critically modulated by the expression of transforming growth factor-beta (TGF-beta), TGF beta can induce the transformation of corneal keratocytes into myo broblasts, which is the main cause of corneal brosis or scar formation [10]
Summary
We downloaded an original microarray data set GSE6676 from the NCBI Gene Expression Comprehensive Database (NCBI-GEO) and analyzed to obtain DEGs between mice that have the overexpression of TGF beta and wildtype litter mate to the mice that have the overexpression of TGF beta. There are no researches about di erential gene analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analyses, and protein-protein interaction (PPI) network analyses of corneal scar using the dataset of GEO database. MMP9 (matrix metallopeptidase 9), CXCL10 (C-X-C motif chemokine ligand 10), MAPK8 (mitogen-activated protein kinase 8), TLR4 (toll-like receptor 4), HGF (hepatocyte growth factor), EDN1 (endothelin 1) were selected as hub genes.
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