Abstract
Hair follicle stem cells (HFSCs) have been shown to be essential in the development and regeneration of hair follicles (HFs). The Inner Mongolia Cashmere goat (Capra hircus) has two types of HFs, primary and secondary, with cashmere being produced from the secondary hair follicle. To identify the genes associated with cashmere growth, transcriptome profiling of anagen and telogen secondary HFSCs was performed by RNA-Seq. The RNA-Seq analysis generated over 58 million clean reads from each group, with 2717 differentially expressed genes (DEGs) detected between anagen and telogen, including 1500 upregulated and 1217 downregulated DEGs. A large number of DEGs were predominantly associated with cell part, cellular process, binding, biological regulation and organelle. In addition, the PI3K-Akt, MAPK, Ras and Rap1 signaling pathways may be involved in the growth of HFSCs cultured in vitro. The RNA-Seq results showed that the well-defined HFSC signature genes and cell cycle-associated genes showed no significant differences between anagen and telogen HFSCs, indicating a relatively quiescent cellular state of the HFSCs cultured in vitro. These results are useful for future studies of complex molecular mechanisms of hair follicle cycling in cashmere goats.
Highlights
Hair follicles, as skin appendages, play crucial role in skin homeostasis, and are important in thermal regulation, social communication and sensory processes
Immunocytochemistry staining showed that Krt15, Krt19 and Sox9 were positively expressed in the ana-SHFSCs and tel-SHFSCs (Fig 2)
We identified a set of keratins (KRT) and keratin-associated protein (KRTAP) genes that were annotated in cashmere goat (Capra hircus) hair follicles [24] (Table 3)
Summary
As skin appendages, play crucial role in skin homeostasis, and are important in thermal regulation, social communication and sensory processes. In the 1980s, the location and characteristics of hair follicle stem cells were first identified using isotope labeling in mice [1], and subsequent studies have suggested that HFSCs play crucial roles in HF morphogenesis and cycling [2,3]. The results of intensive research in stem cell biology suggest that HFSCs are an excellent cell lineage to study stem cell plasticity [4], since most adult stem cells lose their. Expressed genes in anagen and telogen hair follicle stem cells of cashmere goat. RNA-seq of cashmere goat anagen HFSC, SRA: SRR10417580)
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