Abstract

The formation of carboxylase by living bakers' yeast was demonstrated upon incubation of the yeast with either thiamine or 2-methyl-4-amino-5-ethoxymethylpyrimidine, in the presence and in the absence of glucose. Carboxylase is also formed upon incubation of the yeast with NH 4 sulfate and glucose. In the presence of glucose the effects of thiamine + NH 4 sulfate or 2-methyl-4-amino-5-ethoxymethylpyrimidine + NH 4 sulfate considerably surpass the effects of any of these substances separately. The effect of 2-methyl-4-amino-5-ethoxymethylpyrimidine depends upon the primary formation of thiamine pyrophosphate.

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