Publisher Summary This chapter reviews the enzymatic formation of thiamine pyrophosphate in plants. Thiamine pyrophosphokinase (EC 184.108.40.206) converts thiamine to thiamine pyrophosphate (TPP) through a direct transfer of pyrophosphate from adenosine triphosphate (ATP). The content of TPP formed during the enzyme reaction is manometrically determined by measuring the amount of the carbon dioxide evolved in the decarboxylation reaction in which the substrate, pyruvate, is transferred to acetaldehyde and carbon dioxide by pyruvate decarboxylase as a holoenzyme formed from the coenzyme produced, TPP, and added apoenzyme. The comparable relation between increasing concentrations of TPP and increasing activity of the decarboxylation—that is, the increasing amounts of evolved carbon dioxide—is accomplished under experimental conditions. The chapter describes the properties of the thiamine pyrophosphate obtained from plants.
AI-powered Research feed
Round-ups are the summaries of handpicked papers around trending topics published every week. These would enable you to scan through a collection of papers and decide if the paper is relevant to you before actually investing time into reading it.
Climate change Research Articles published between Jun 20, 2022 to Jun 26, 2022
Jun 27, 2022
Articles Included: 2
One eighth of the bird species in the world is considered globally threatened; the avifauna of Iraq comprises 409 species and is considered as the maj...Read More