Experimental substantiation of estimating the optical density of venous blood plasma to determine intestinal viability

Abstract

The aim to evaluate the possibility of determining the optical density of venous blood plasma (ODVBP) to identify the viability of the intestines in the experiment.Material and methods. For the study, 30 nonlinear white rats were used. In 10 animals, the loop of the middle part of the small intestine was tied with a ligature to simulate destruction, and in 10 animals, the loop of the middle part of the colon was ligated. After 12 h, a laparotomy was performed, the ligated loop of the intestine was removed for histological examination and blood was drawn from the jugular vein to measure ODVBP. The control was given to 10 healthy animals.Results. Healthy animals at a wavelength lambda = 310 nm found a specific maximum ODVBP, reaching 0.22 units (0.22 ± 0.01). After 12 h in animals with models of small bowel destruction, the ODVBP values at this wavelength were 0.38 ± 0.17 units, which was statistically significantly (p <0.01) higher than the control values. In animals with models of colon destruction, the indicators of ODVBP at this wavelength were 0.37 ± 0.13 units, which was statistically significantly (p <0.01) higher than the control values. There were no statistically significant differences between the indicators of animals with models of destruction of different parts of the intestine (p> 0.05). Necrotic changes of the intestinal wall were detected on all histological preparations.Conclusions. 1. According to experimental data, in healthy rats at a wavelength lambda = 310 nm, there is a peak ODVBP, reaching 0.22 ± 0.01 units. 2. In animals with models of destruction of the small intestine, the rate of ODVBP at this wavelength increases to 0.38 ± 0.17 units, and in animals with models of destruction of the colon - up to 0.37 ± 0.13 units, which is statistically significant (p (0.01), exceeds the control values, at the same time there are no statistically significant distinctions in different localization of intestinal destruction (p> 0.05). 3. The data obtained indicate the prospects for clinical use of such an index to assess the condition of the intestines under conditions of their destruction and the need for appropriate clinical trials.