Abstract
The aim of this study was to investigate the effectiveness of hydatid cyst toxin fractions in mice. Fifty male mice were divided into five groups with 10 mice for each group the first group as control the second group was injected with toxin fractions at the concentrations 25Mg/ml PBS, the third group are the mice that injected intraperitoneally with toxin fractions at the concentrations 25Mg/ml PBS and treated with vitamin E at the concentration of 40 mg/ 100 g of feed, the fourth group was injected intraperitoneally with 1 ml of raw fluid and the fifth group was injected intraperitoneally of 1ml of row fluid with the vitamin E at the concentration of 40 mg /100gm feed. The mice were sacrificed after 15 and 30days post injection, specimen of heart are fixed in 10% neutral buffered formalin for histological techniques. The histopathological changes in cardiomyocyte were edema, infiltration of mononuclear cell and thickening of blood vessels wall with congestion in it. The results confirm that the toxin fraction have more effect than the raw fluid and that there is the regeneration effect of vitamin E on hydatid cyst cardiomyocyte.
Highlights
Hydatid cyst is a zoonotic helminthic malady caused by Echinococcus granulosus parasite, which is distributed in the Middle East [1,2] in the stage of larva
Fifty male mice were divided into five groups with 10 mice for each group the first group as control the second group was injected with toxin fractions at the concentrations 25Mg/ml phosphate buffer saline (PBS), the third group are the mice that injected intraperitoneally with toxin fractions at the concentrations 25Mg/ml PBS and treated with vitamin E at the concentration of 40 mg/ 100 g of feed, the fourth group was injected intraperitoneally with 1 ml of raw fluid and the fifth group was injected intraperitoneally of 1ml of row fluid with the vitamin E at the concentration of 40 mg /100gm feed
This paper studied the effect of toxin fractions of hydatid cyst fluid on the mice myocardial muscle sections which stained with HE
Summary
Hydatid cyst is a zoonotic helminthic malady caused by Echinococcus granulosus parasite, which is distributed in the Middle East [1,2] in the stage of larva. The Echinococcus granulosus larva go along from the gastrointestinal via the portal system to the, liver, lung and other organs, they are converted to cyst leading the serious effects on these organs [3,4]. The hydatid cyst in lung were more fertile 55% in contrast to liver cysts 45%. The wall of the cyst formed from the internal germinal layer, the middle laminated/lamellate layer, and the outer layer consist of fibers. Inflammatory response occur around the hydatid cyst was changeable and was recognized by loose regulated of mononuclear infiltrated with fibroblasts cells densely regulated mononuclear along with fibroblasts cells; and an external layer of fibrous connective tissue. Fibroplasia and Calcification were distinguishing at this site [6]
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