Experimental study on gastroprotective efficacy and mechanisms of luteolin-7-O-glucoside isolated from Ophiorrhiza mungos Linn. in different experimental models

Abstract

The gastroprotective effect of luteolin-7-O-glucoside (LUT7G) on different ulcer models using rats including the indomethacin model, the ethanol-induced gastric ulcer model and the Shay ulcer model was examined. The ethanol-induced ulcer group showed significant increases in MPO, NO, iNOS, MMP-2, MMP-9, caspase-3, apoptosis, IL-6, TNF-α, IKK, NF-κB p65, and ICAM-1 and declines in PGE2, arginase, SOD, GSH, mucin, IL-10, eNOS, COX-1, HSP-70, and IκBα levels. However, LUT7G (25 mg/kg) pretreatment significantly reverted the pathophysiological levels of these biomarkers to near normal levels. The gastroprotective activity of LUT7G was abolished by pretreatment with SC560, rofecoxib, and L-NAME, demonstrating the participation of COX and NOS in LUT7G-facilitated gastroprotection against ethanol-induced ulcers. Convincingly, LUT7G (25 mg/kg) provided protective effects in the rat gastric mucosa against ethanol-induced gastric injury at least in part to antisecretory, anti-inflammatory, antioxidative, and antiapoptotic activity, and augmentation of PGE2, mucin and HSP-70 synthesis.