The ameliorative effect of fisetin, a bioflavonoid, on ethanol-induced and pylorus ligation-induced gastric ulcer in rats

Abstract

Fisetin, a tetrahydroxy flavone, exhibits many biological activities such as antioxidant, antibacterial and anti-inflammatory. The aim of present study was to unravel the therapeutic potential of fisetin at a dose of 10, 20, 30 mg/kg, per oral (p.o.), in ethanol-induced gastric ulcer and pylorus ligation-induced gastric ulcer. Omeprazole (20 mg/kg) was used as a standard drug. In ethanol-induced ulcer, after the pretreatment period of 1 hr gastric ulcer was induced with absolute ethanol at a dose of 8 ml/kg (p.o.), where as in pylorus ligation-induced gastric ulcer; after the pretreatment period of 1 hr ulcer was induced by tight ligation of pylorus portion of stomach. In the pylorus ligation-induced ulcer model there was a significant reduction in the ulcer area as well as the total volume, free acidity and total acidity and increase in the pH of gastric content along with the mucous production were found. There was a significant decrease in ulcer area and significant increase in the mucosal production in the ethanol-induced gastric ulcer model. Fisetin significantly lowered the level of lipid peroxidase, neutrophil infiltration along with gastric mucosal nitrite in both models of the gastric ulcer. The present findings elucidate the therapeutic value of fisetin in the prevention of experimental gastric ulcer by virtue of its antioxidant mechanism. Key words: Antioxidant, antiulcer, fisetin, lipid peroxidation, nitric oxide