Experimental study on anti-tumor effect of high intensity focused ultrasound ablation combined with liquid fluorocarbon nanoparticle on cervical cancer

Abstract

Objective To investigate the therapeutic effect of high intensity focused ultrasound(HIFU) ablation combined with homemade liquid fluorocarbon nanoparticles on cervical cancer in nude mice. Methods The cell experiment was divided into three groups: a, control group; b, HIFU group; c, HIFU+ PFB nanoparticle group, and the viability of cells was detected using CCK-8 reagent. The mice were also divided into three groups: A, 0.9%NaCI group; B, HIFU+ 0.9%NaCI group; C, HIFU+ PFB nanoparticle group. The tumors were removed and underwent triphenyl tetrazolium chloride(TTC) staining, and the necrosis area was measured. Histopathological changes of the tumors were examined by light microscopy. Results After HIFU irradiation, the viability rate of group c was (40.5±9.7)%, it was lower than that of group b (77.7±8.5)% (P<0.05) and that of group a(100±4.8)% (P<0.05). TTC staining of tumor showed a large scale of necrotic tissue in group C. The necrosis ratio of the three groups was 0%, (34.14±12.2)% and (65.97±25.1)%, respectively (P<0.05). HE staining showed karyorrhexis or an absence of nuclei in group B and group C, which demonstrated the coagulation necrosis. Conclusions HIFU ablation combined with liquid fluorocarbon nanoparticles can effectively treat the xenograft model of the human cervical carcinoma in nude mice. Key words: High intensity focused ultrasound; Nanoparticles; Uterine cervical neoplasms; Liquid fluorocarbon