Experimental study of the cream with cerium dioxide nanoparticles on the model of photodynamic injury in guinea pigs

Abstract

Aim. To study the photoprotective action of the cream with cerium dioxide nanoparticles (CDN) on the model of the photodynamic injury in guinea pigs.Materials and methods. CDN were synthesized in OOO “NanoMedTech”, the cream with CDN was developed in SSI “Institute for Single Crystals” of the NAS of Ukraine. The photodynamic injury in guinea pigs was modeled with an UV-emitter. The cream with CDN was applied preventively in the dose of of 2 mg/cm2. The erythema intensity was assessed according to S. V. Suvorov colorimetric scale, then the photoprotective activity (PPA) was calculated. The skin temperature was measured within 4 hours after exposure. The wound healing action was assessed as the number of days till complete healing of the skin of guinea pigs.Results and discussion. The photoprotective action (PPA – 43.2 %) of the cream with CDN exceeded that of the reference drug (the cream with titanium dioxide) since the number of ulcers and deep lesions of the skin was lower. The preventive application of the cream with CDN led to the skin temperature normalization, which confirmed the ability of CDN to prevent inflammation. The wound healing action of the cream was also observed – the complete epithelization of the damaged zone took place in 5.86 days compared to 11.00 days in untreated animals.Conclusions. The results regarding the photoprotective, wound healing action of the cream with CDN and its ability to prevent inflammation create opportunities for further study of this formulation as a photoprotector.