Abstract
Nystatin (nys) is an antifungal agent that preferentially forms ion channels in membranes containing ergosterol (erg) or cholesterol (chol). When vesicles containing nys and erg fuse with a sterol-free bilayer, characteristic spike changes in membrane conductance are observed, which result from nys/erg channels transported to the bilayer by the fusion of vesicles with the bilayer. We previously studied the decay of these conductance spikes when the mol fraction of erg in the vesicles was chosen to produce specific superlattices in vesicle membranes. Here we report the results of similar experiments in which vesicles containing nys and chol were made to fuse with phospholipid bilayers. We observe conductance spikes, which decay spontaneously after long periods of sustaining constant currents. Fusion experiments with erg rather than chol, but otherwise identical conditions continue to produce decaying spikes.
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