EXPERIMENTAL JUSTIFICATION OF DEVELOPMENT OF MULTIPLE ORGAN FAILURE SYNDROME BASED ON THE DYNAMICS OF MORPHOLOGICAL CHANGES IN INTERNAL ORGANS WITH ACUTE DIFFUSE PERITONITIS

Abstract

An urgent problem of urgent abdominal surgery remains acute diffuse peritonitis. Mortality rate with acute diffuse peritonitis is quite high due to development of multiple organ failure syndrome. The objective of the study is to investigate the dynamics of morphological changes in the internal organs in animals with simulated acute diffuse peritonitis depending on the stage of the inflammatory process of the peritoneum. Materials and Methods. Acute peritonitis was modeled on 48 nonlinear rats according to V.A. Lazarenko’s method. Both, experimental and control groups included 24 animals. Animals were taken from the experiment on the 1, 3 and 7 days after simulated peritonitis. Results and conclusions: on the first day of modeling peritonitis histological examination of the peritoneum found the following in animals of the main group: focal purulent peritonitis with parietal peritoneal involvement, diffuse hyperemia, and focal edema of the connective tissue. Morphological and functional changes in the liver and kidneys were not found. However, on the third day of the study, the structure of the liver lobe was preserved only partially. The central veins were visualized well. Some of them were dilated and contained a small amount of red blood cells. Changes in the pulmonary tissue: enlarged alveoli, hyperemia of small vessels and hemorrhages. On the seventh day the structure of the liver lobe was significantly disturbed. The central veins were well visualized, slightly enlarged and contained a large number of red blood cells. Sinusoids were visualized in most fields of vision. Sometimes only central lobular ones were seen. The balloon organization of hepatocytes was disturbed throughout the size of the lobe. Histological examination of the kidney tissues of animals on the 7th day of the experiment in the cortical layer of the kidney found the following: the glomeruli were somewhat enlarged, vessels were filled with blood, serous exudates were observed in the lobes, and certain glomeruli were wrinkled. The vessels of the venous circulation were dilated and full of blood. Small perivascular dotted hemorrhages were visualized.