Experimental evidence that refuges delay insect adaptation to Bacillus thuringiensis

Abstract

Restricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Liu Yong-Biao and Tabashnik Bruce E. 1997Experimental evidence that refuges delay insect adaptation to Bacillus thuringiensisProc. R. Soc. Lond. B.264605–610http://doi.org/10.1098/rspb.1997.0086SectionRestricted accessExperimental evidence that refuges delay insect adaptation to Bacillus thuringiensis Yong-Biao Liu Yong-Biao Liu Department of Entomology, University of Hawaii, Honolulu, Hawaii 96822 [email protected] Google Scholar Find this author on PubMed Search for more papers by this author and Bruce E. Tabashnik Bruce E. Tabashnik Department of Entomology, University of Hawaii, Honolulu, Hawaii 96822 Google Scholar Find this author on PubMed Search for more papers by this author Yong-Biao Liu Yong-Biao Liu Department of Entomology, University of Hawaii, Honolulu, Hawaii 96822 [email protected] Google Scholar Find this author on PubMed Search for more papers by this author and Bruce E. Tabashnik Bruce E. Tabashnik Department of Entomology, University of Hawaii, Honolulu, Hawaii 96822 Google Scholar Find this author on PubMed Search for more papers by this author Published:22 April 1997https://doi.org/10.1098/rspb.1997.0086AbstractTheoretical projections suggest that refuges from exposure can delay insect adaptation to environmentally benign insecticides derived from Bacillus thuringiensis, but experimental tests of this approach have been limited. We tested the refuge tactic by selecting two sets of two colonies of diamondback moth (Plutella xylostella) for resistance to B. thuringiensis; subsp. aizawai in the laboratory. In each set, one colony was selected with no refuge and the other with a 10 per cent refuge from exposure to B. thuringiensis subsp. aizawai. Bioassays conducted after nine selections were completed show that mortality caused by B. thuringiensis subsp. aizawai was significantly greater in the refuge colonies than in the no–refuge colonies. These results demonstrate that the refuges delayed the evolution of resistance. Relative to a susceptible colony, final resistance ratios were 19 and 8 for the two no–refuge colonies compared to 6 and 5 for the refuge colonies. The mean realized heritability of resistance to B. thuringiensis subsp. aizawai was 0.046 for colonies without refuges, and −0.002 for colonies with refuges. Selection with B. thuringiensis subsp. aizawai decreased susceptibility to B. thuringiensis toxin Cry1Ab, but not to Cry1 C or B. thuringiensis subsp. kurstaki Although the ultimate test of refuges will occur in the field, the experimental evidence reported here confirms modelling results indicating that refuges can slow the evolution of insect resistance to B. thuringiensis Previous ArticleNext Article VIEW FULL TEXT DOWNLOAD PDF FiguresRelatedReferencesDetailsCited by Morishita M (2019) High-Dose/Refuge Strategy for Insect Resistance to Bt Crops害虫のBt作物抵抗性に対する高濃度/保護区戦略, Japanese Journal of Applied Entomology and Zoology, 10.1303/jjaez.2019.29, 63:2, (29-38), Online publication date: 25-May-2019. 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