Abstract
The shape of nonlinear elution peaks was studied experimentally in various chromatographic modes and a wide sample size range encompassing the case where the distribution isotherm curvature is the prevailing factor of band broadening. It is shown that as sample size is varied, the tailing part of all peaks adjoins a common curve that can be fitted with an exponential function whose parameters are independent of mobile phase linear velocity and stationary phase particle size. Preparative liquid chromatography (PLC) often needs merely a rough understanding of the nonlinear behavior of the compounds to be separated in order to reduce the number of preliminary injections necessary for optimizing the injected volume and concentration of the sample. PLC often needs merely a rough understanding of the nonlinear behavior of the compounds to be separated in order to reduce the number of preliminary injections necessary for optimizing the injected volume and concentration of the sample.
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