Abstract
The purpose of this study was to evaluate the development of candidosis in rat’s tongue after intraepithelialinjections of Candida albicans. Fifty rats (Rattus norvegicus, Albinus, Wistar), originally negative for the Candidaspp. received ten intraepithelial injections of C. albicans on the dorsal tongue. Groups of five animals werekilled after 1, 2, 4, 6, 8, and 12 hours and 1, 2, 7, and 15 days after the injection. The rat’s tongues weresurgically removed and then macroscopic and microscopic analyses were performed. The development of candidosislesions was observed in all the rats studied. One hour after the injection, the development of germ tubesfrom the yeast cells could be observed. After 4 hours, Candida spp. pseudohyphae penetrated the epithelial cellswith the formation of microabscesses. After 24 to 48 hours, the epithelial areas with pseudohyphae invasionpresented desquamation, hyperplasia of the basal layer and discrete inflammation of the connective tissue. Afterseven days, few pseudohyphae could be observed. The epithelium presented acanthosis, hyperkeratosis and lossof filiform papillae. After 15 days, neither yeasts nor pseudohyphae were found. In some areas, the epitheliumpresented acanthosis and loss of filiform papillae. It can be concluded that the intraepithelial injection of Candidaalbicans on the dorsal rat tongue caused candidosis lesions in all the animals studied. C. albicans waspresent until seven days after the injections.
Highlights
Candida albicans is the most common and potentially invasive fungus of the human oral cavity
The purpose of this study was to observe the development of candidosis induced by intraepithelial injections of C. albicans in the dorsal rat tongues
The intraepithelial injection of a suspension of C. albicans cells in the dorsal rat tongue induced macroscopic lesions and microscopic alterations in the epithelium as well as in the connective tissue subjacent to the inoculated area. These experiment findings are in accordance to previous studies that proved that the oral inoculation of C. albicans is capable of producing lesions in the mucosa of rats[5, 6, 11, 12, 17]
Summary
Candida albicans is the most common and potentially invasive fungus of the human oral cavity. The first experimental model developed with the purpose of examining Candida colonization on rat’s tongue concluded that 55% of the animals studied presented infection by this fungus. These studies showed that the mycelium forms did not penetrate underneath the stratum corneum and were associated with the loss of lingual papillae, presence of hyperkeratosis in the epithelium, accumulation of mononuclear cells and alterations on the most superficial muscle layer[10]
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