Detection and identification of morphine in blood of male white rats (rattus norvegicus) by ultraviolet-visible spectrophotometry

Abstract

The blood of male white rats has been experimentally characterized by Ultraviolet-visible (UV-Vis) Spectrophotometry. Ultraviolet-visible (UV-Vis) spectrophotometry appears as an affordable option to monitor morphine composition in blood of male white rats (Rattus norvegicus). Morphine compounds were identified following two different methods, namely qualitative and quantitative methods. Qualitative analysis of this research by the marquis test and quantitative analysis with spectrophotometry method. The male white rats (Rattus norvegicus) received morphine and it was maintained drug free for 10 days. After that, the blood was taken from the male white rats (Rattus norvegicus) and were extracted the blood using organic solvents, namely methanol and chloroform (1: 1). In the extraction process was used sonication (42 KHz) at temperature 500°C. The qualitative test proves the presence of morphine content with respective concentrations of 1%, 2%, 3%, 4% and 5%. Furthermore, identification of ultraviolet-visible (UV-Vis) spectrophotometry showed that morphine levels in each subcutaneous, that is 0%; 0.6%; 1.0%; 1.6% and 3.5%. The percent transmittance on each samples are 99.9%; 76.9%; 68.8%; 55.9% and 31.0%. The successful application of this simple methodology to a blood of male white rats (Rattus norvegicus) suggests that this approach has practical utility for confirming the identity of abused drugs like morphine detected by ultraviolet-visible (UV-Vis) spectrophotometry.