Experimental application of a split-conjugated vaccine against animal brucellosis in small cattle

Abstract

A study was made of the blood samples of small cattle for brucellosis with various serological reactions when testing a split-conjugated vaccine against animal brucellosis three and a half, four and a half months and nine months after immunization. Positive reaction of small cattle to brucellosis in the complement fixation reaction and the agglutination reaction of 40.7% of sheep and goats, and 44.4% of animals in Ros-Bengal after 3.5 months of vaccination. The vaccine has a high immunogenicity against goats, a high enough antibody titer in blood three and a half months after vaccination, when tested with agglutination and Rose-Bengal, breakdown was detected in 83.3% of animals, and in the complement fixation reaction it was 66.7%, which will, in our opinion, effectively use this vaccine in the fight against goat brucellosis. Immunized sheep in the complement-fixation reaction and Rose-Bengal assay reacted positively only in 33.3% of cases. When the agglutination reaction was established three and a half months after the introduction of the vaccine - in 28.6% of the animals, in connection with the data obtained, we recommend looking for new immunoprotectors capable of enhancing the humoral immune response of sheep to the antigen of the tested anti-brucellosis vaccine. Nine months after the vaccination of the split-conjugated vaccine in all blood samples of small cattle (sheep and goats) with all of the above reactions, there was no positive test, which indicates the disappearance and complement-binding and agglutinating antibodies from the bloodstream of animals, and thus the opportunity is given differentiate postvaccinal antibodies in the blood serum of animals, from post-infection, in the case of their detection within the time specified above.