Evaluation of Humoral Immune Responses of Sheep Vaccinated With Razi Institute Rev.1 Brucellosis Vaccine in Comparison to a Spanish (CZV) Rev.1 Vaccine

Abstract

Background: Prevention of brucellosis in humans is based on the vaccination of animals. Given that Rev.1 vaccine is one of the most effective vaccines for preventing and controling brucellosis in sheep and goats, the present study was conducted to evaluate and compare humoral immune responses of sheep against Razi Institute and Spanish (CZV) Rev.1 brucellosis vaccines. Methods: To do the study, 6 sheep were prepared and divided into 2 groups, and blood samples were then collected on day zero. The animals of each group were subcutaneously vaccinated with one dose of Razi Institute and Spanish (CZV) Rev.1 vaccines followed by collecting blood samples on days 30, 60, 90, 120, 150, and 180 post vaccination. Sheep serum samples were then tested using Rose Bengal, Wright, and 2-Mercaptoethanol assays and the data were statistically analyzed. Results: The results showed that the highest titers of Wright and 2-Mercaptoethanol tests were observed 30 days after vaccination. However, no statistically significant difference was observed between humoral immune responses of sheep vaccinated with either Razi Institute or Spanish (CZV) Rev.1 vaccines (P>0.05). Conclusions: Given the similar results of both vaccines in stimulating the humoral immune system in the target species and the indigenous Razi vaccine production technology, as well as its lower price compared to the imported one, this native vaccine can certainly be used for immunizing livestock in our country. This can ensure the country’s independence, boost national vaccine production, and prevent the outflow of currency