Experimental and Theoretical Analysis of Metal Complex Diffusion through Cell Monolayer.

Katarzyna Gałczyńska,Jacek Semaniak,Karol Ciepluch,Magdalena Kowalska,Michał Arabski,Sławomir Wąsik,Krystyna Kurdziel,Katarzyna D Lewandowska,Jarosław Rachuna,Tadeusz Kosztołowicz

doi:10.3390/e23030360

Katarzyna Gałczyńska, Jacek Semaniak + Show 8 more

Open Access

https://doi.org/10.3390/e23030360

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Abstract

The study of drugs diffusion through different biological membranes constitutes an essential step in the development of new pharmaceuticals. In this study, the method based on the monolayer cell culture of CHO-K1 cells has been developed in order to emulate the epithelial cells barrier in permeability studies by laser interferometry. Laser interferometry was employed for the experimental analysis of nickel(II) and cobalt(II) complexes with 1-allylimidazole or their chlorides’ diffusion through eukaryotic cell monolayers. The amount (mol) of nickel(II) and cobalt(II) chlorides transported through the monolayer was greater than that of metals complexed with 1-allylimidazole by 4.34-fold and 1.45-fold, respectively, after 60 min. Thus, laser interferometry can be used for the quantitative analysis of the transport of compounds through eukaryotic cell monolayers, and the resulting parameters can be used to formulate a mathematical description of this process.

Highlights

Transport of substances through biological membranes plays a crucial role in many physiological processes and influences the effectiveness of drug-based therapies
Metal complexes with imidazole derivatives have anticancer, anti-inflammatory, antibacterial, antifungal and antiviral properties [43,44,45,46,47,48,49]
The laser interferometry method was optimized for the quantitative analysis of the transport of compounds across a cell monolayer as a model tissue

Summary

Introduction

Transport of substances through biological membranes plays a crucial role in many physiological processes and influences the effectiveness of drug-based therapies. Various theoretical models of molecule transport through membrane channels have been proposed [2,8,9,10,11,12,13]. The experimental testing of the validity of these models helps to better understand membrane transport processes. This facilitates the design of synthetic membranes with increased permeability [3], and the development and optimization of more complicated processes such as controlling the flux of macromolecules through membrane proteins [6,14]. The optimization of diffusion diffusive transport through the cell membrane is important for the design of molecules intended for clinical use

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