Experimental and numerical study on the performance of printed alginate/hyaluronic acid/halloysite nanotube/polyvinylidene fluoride bio-scaffolds

Abstract

The growing usage of printed bio scaffolds in the field of regenerative medicine has made this field very important in biomedical engineering. In this regard, three-dimensional printing (3D) technique needs bio-materials with higher mechanical and biological performance. The biomaterials with high mechanical performance beside its bio compatibility are limited. A novel bio-material made of Alginate, Hyaluronic acid, Halloysite Nanotube and Polyvinylidene Fluoride was used and characterized for printing cartilage bio scaffolds through numerical studies. CaCl2 was used for crosslinking of biomaterial. Scanning Electron Microscopy, mechanical tests (tensile and compressive test), MTT assay were conducted for evaluating this novel biomaterial. Different structures of bio material were simulated for numerical studies. The numerical study was performed in ANSYS 18 using three parameter Mooney–Rivlin model. According to experimental and numerical results, Halloysite Nanotube increases the tensile and compressive strength of biomaterial up to 47%. Results show that biomaterial have good mechanical performance due to mechanical forces required for cartilage bio scaffolds besides its high biological performance. Polyvinylidene fluoride reduces the mechanical performance while increasing the cell viability. MTT assay results performed on day 0, day 2 and day 6 show increase in cell number to be about twice for biomaterial containing 40 mg/ml alginate, 40 mg/ml halloysite nanotube, 10 mg/ml hyaluronic acid and 1 w/v Polyvinylidene fluoride. Numerical simulation shows high mechanical performance of bio material in different scaffolds structure. The best structure of bio scaffolds was achieved with 0.4 mm nozzle diameter and 0.4 space between rows.