Abstract
BackgroundThe current study reports dissemination of highly stable blaOXA-10 family of beta lactamases among diverse group of nosocomial isolates of Gram-negative bacilli within a tertiary referral hospital of the northern part of India.MethodsIn the current study, a total number of 590 Gram negative isolates were selected for a period of 1 year (i.e. 1st November 2011–31st October 2012). Members of Enterobacteriaceae and non fermenting Gram negative rods were obtained from Silchar Medical College and Hospital, Silchar, India. Screening and molecular characterization of β-lactamase genes was done. Integrase gene PCR was performed for detection and characterization of integrons and cassette PCR was performed for study of the variable regions of integron gene cassettes carrying blaOXA-10. Gene transferability, stability and replicon typing was also carried out. Isolates were typed by ERIC as well as REP PCR.ResultsTwenty-four isolates of Gram-negative bacilli that were harboring blaOXA-10 family (OXA-14, and OXA16) with fact that resistance was to the extended cephalosporins. The resistance determinant was located within class I integron in five diverse genetic contexts and horizontally transferable in Enterobacteriaceae, was carried through IncY type plasmid. MIC values were above break point for all the tested cephalosporins. Furthermore, co-carriage of blaCMY-2 was also observed.ConclusionMultiple genetic environment of blaOXA-10 in this geographical region must be investigated to prevent dissemination of these gene cassettes within bacterial population within hospital settings.
Highlights
The current study reports the dissemination of highly stable blaOXA-10 family among diverse group of nosocomial isolates of Gram-negative bacilli within a tertiary referral hospital of the northern part of India
A total of 58.5% (n = 345) isolates were resistant to expanded-spectrum cephalosporins
In case of Enterobacteriaceae, minimum inhibitory concentration (MIC) of donor strain and transformants were observed above the breakpoint against cephalosporins, carbapenems, and monobactams (Table 2) and similar MIC pattern was too observed in P. aeruginosa (Table 3)
Summary
The current study reports dissemination of highly stable blaOXA-10 family of beta lactamases among diverse group of nosocomial isolates of Gram-negative bacilli within a tertiary referral hospital of the northern part of India. The ever-increasing use of antibiotics with the evolution of intrinsic and Maurya et al BMC Res Notes (2017) 10:145 two decades restricting therapeutic options. These genes are often reported to be located within integron gene cassettes [3, 4]. The current study reports the dissemination of highly stable blaOXA-10 family among diverse group of nosocomial isolates of Gram-negative bacilli within a tertiary referral hospital of the northern part of India
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
