Abstract
Although significant clinical advances have been made in the treatment of cancer using the immune system, discovery of therapeutic cancer vaccines still remains as an area of interest. Development of the method of pulsing dendritic cells with tumor antigens set the stage for the development of cancer vaccines. Exosomes have gained significant interest because of their ability to activate dendritic cells to recognize and kill cancerous cells. Because of their characteristics such as superior biosafety profile to other nanoparticles, exosomes are promising nanocarriers for clinical use, which makes them an attractive candidate for cancer vaccine development. Identification of novel vaccinations for immunoprevention can be studied by exosomes. This chapter describes commonly used methods to isolate and manipulate exosomes.
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