Abstract
The aim of this research is to estimate the virulence factor L. major exosome by real time gene expression. The higher gene expression in footpad tissue was (29.34), the higher gene expression in day seven culture was (20.37), higher gene expression in day twelve culture was (12.25), highest gene expression in culture line control (8.33). The current work revealed that exosome gene expression was elevated in footpad in comparison to cultures media. The elevated levels were in passage three and lowest levels in passage one, in control culture line the level of gene expression was declined with progress of time.
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