Exogenously applied calcium regulates antioxidative system and reduces cadmium-uptake in Fagopyrum esculentum

Abstract

Calcium (Ca) being macronutrient plays a prominent role in signal transduction during various abiotic stresses. However, their involvements to alleviate heavy metal stress in plants remain evasive. In the present investigation, we found that application of exogenous Ca to Cd-stressed common buckwheat plants reversed the toxic effects of Cd by enhancing root and shoot length, biomass accumulation and reduced Cd-uptake as revealed by the translocation factor (<1), indicating more Cd is restrained in the roots. Moreover, present data also revealed that exogenous Ca significantly alleviated the Cd-induced oxidative damage by enhancing proline by 66.12% and 47.20% respectively in roots and shoots than control. The decline in the total chlorophyll content upon Ca application in Cd-treated plants was found less (38.96%) compared to buckwheat plants treated with Cd-stress alone (80.2%). APX and POD activities increased by 1.97 and 1.44 times in shoots, respectively, and increased by 2.81and 1.33 times in roots, respectively compared to the Cd-treated plants alone. The mineral content (Ca, K, Mg, Fe, P and S) that were suppressed in Cd-treated plants in both root and shoot were restored upon exogenous Ca application. Further, the correlation analysis showed significant positive correlation among proline and GSH synthesis in the Ca + Cd treatment. The correlations of Ca revealed to be positive with enhanced levels of APX and POD activity. Our data showed that exogenous application of Ca minimizes the Cd-toxicity and modulates the physiological and biochemical pathway in common buckwheat to withstand Cd-induced oxidative stress.