Exogenous application of IAA alleviates effects of supplemental ultraviolet-B radiation in the medicinal plant Withania somnifera Dunal.

Abstract

Supplemental (s)-UV-B radiation has adverse effects on the majority of plants. The present study was conducted to evaluate the effects of exogenous application of the growth hormone indole acetic acid (IAA) on various morphological, physiological and biochemical characteristics of Withania somnifera, an indigenous medicinal plant, subjected to s-UV-B. The s-UV-B-treated plants received ambient+3.6kJm-2 ·day-1 biologically effective UV-B, and IAA was applied at two doses (200 and 400ppm) to s-UV-B-exposed plants. The plant was forced to compromise its growth, development and photosynthetic patterns to survive under s-UV-B by increasing concentrations of secondary metabolites and antioxidants (thiol, proline, ascorbic acid, α-tocopherol, ascorbate peroxidase, catalase, glutathione reductase, peroxidase, polyphenol oxidase, superoxide dismutase) to counteract oxidative stress. Increases in secondary metabolites were evidenced as increased activity of phenylpropanoid pathway enzymes: phenylalanine ammonia lyase, cinnamyl alcohol dehydrogenase, 4-coumarate CoA ligase, chalcone isomerase and dihydroflavonol reductase. Application of different IAA doses reversed the detrimental effects of s-UV-B on W.somnifera by improving growth and photosynthesis and reducing concentrations of secondary metabolites and non-enzymatic antioxidants. Antioxidant enzymes, however, had a synergistic effect on s-UV-B treatment and IAA application. The effects of s-UV-B on W.somnifera are ameliorated to varying degrees upon exogenous IAA application, and synergistic enhancement of antioxidant enzymes under s-UV-B+IAA treatment might be responsible for the partial recuperation of growth and plant protein content, as a UV-B-exposed plant is forced to allocate most of its photosynthate towards production of enzymes related to antioxidant defence.