Exogenous 24-epibrassinolide enhanced benzene detoxification in Chlorophytum comosum via overexpression and conjugation by glutathione

Abstract

Benzene, a hydrophobic xenobiotic, induces cell damage in both humans and plants. Due to its volatilization, benzene is an airborne environmental problem. The potential of an exogenous bioactive brassinosteroid phytohormone to enhance benzene removal for phytoremediation was investigated. Chlorophytum comosum had higher brassinosteroids content under benzene stress. Plant treated with 24-epibrassinolide (EBR) removed significantly more gaseous benzene than untreated plants under both light and dark conditions at an initial benzene of 12.75 μmol in the systematic chambers (P < 0.05). Although benzene increased malondialdehyde in plant tissue, EBR-treated plants lowered this lipid peroxidation by enhancing their antioxidant content and increasing benzene detoxification-related genes expression, including ascorbic acid (AsA), homogentisate phytyltransferase (HPT), and glutathione synthethase (GS). This contributed to maintaining higher photosynthetic performances. Moreover, EBR-treated plants had higher gene expression of ferredoxin–NADP reductase (FNR) and glucose-6-phosphate 1-dehydrogenase (G6PDH), thus promoting NADPH biosynthesis to cope with benzene under light and dark conditions, respectively. Further, higher glutathione biosynthesis promoted more glutathione conjugate of benzene products including S-phenylcysteine (SPC) in EBR-treated plants. Hence, application of exogenous EBR as foliar spray provided for enhanced benzene detoxification via antioxidant content, benzene detoxification-related genes and benzene conjugation products with glutathione (GSH) and consequently greater gaseous benzene removal.