Abstract
Chromatin remodelling plays a critical role in controlling gene expression in eukaryotic cells. Histone deacetylases (HDACs) and histone acetyl transferases (HATs) control the acetylation state of histones and thus regulate the access of transcriptional regulators to DNA. The activity of these enzymes appears to be regulated by their cellular localisation. An acute bout of exercise is known to enhance the expression of several metabolic genes, including GLUT-4. The transcription factor myocyte enhancer factor 2 (MEF2) is required for GLUT-4 gene expression and is also repressed by HDAC5, suggesting that the cellular localisation of HDAC5 could be modulated by exercise. PURPOSE To determine if the nuclear abundance of HDAC5 in skeletal muscle is decreased following an acute bout of exercise in humans. METHODS Seven male subjects (27 ± 3 yrs; 83 ± 4 kg; VO2peak = 47 ± 2 ml·kg-1.min-1) performed 60 min of cycling at 74 ± 2% of VO2peak. Samples of the vastus lateralis were obtained before and immediately after exercise and nuclear proteins were isolated to immunoblot for HDAC5 abundance. Mean pre and post exercise nuclear HDAC5 abundance was compared using a two-tailed t-test. RESULTS Following 60 min of exercise nuclear HDAC5 content was reduced by 54 ± 13% (p < 0.05). CONCLUSION The nuclear export of HDAC5 in response to exercise describes a potential mechanism by which exercise-induced gene expression is regulated.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call