Exendin-4 increases extracellular superoxide dismutase expression in cultured astrocytes

Takano K,Komada Y,Nakamura Y,Moriyama M,Kawabe K

doi:10.15761/gdt.1000s1001

Abstract

Exendin-4 increases extracellular superoxide dismutase expression in cultured astrocytes

Highlights

Exendin (Ex)-4 is a peptide found from saliva of Gila monster (Heloderma suspectum) consisting of 39 amino acids, an analog of glucagon-like peptide-1 (GLP-1), and an agonist of GLP-1 receptor [1,2]
Cultured astrocytes were stimulated with 10 nM Exendin 4 (Ex-4) for indicated time, and the mRNA expressions of EC-superoxide dismutase (SOD) were analyzed by realtime Reverse transcription-polymerase chain reaction (RT-PCR)
The expression of EC-SOD mRNA was significantly increased by 10 nM Ex-4 for 3 h exposure, it was remarkably increased at 6 h and the increased level was sustained up to 24 h (Figure 1)

Summary

Introduction

Exendin (Ex)-4 is a peptide found from saliva of Gila monster (Heloderma suspectum) consisting of 39 amino acids, an analog of glucagon-like peptide-1 (GLP-1), and an agonist of GLP-1 receptor [1,2]. In the central nervous system (CNS), SOD isozymes are expressed and there are many reports suggesting that SOD plays important roles in defense against hypoxia-induced brain injury and neurodegenerative diseases such as PD [28,29,30,31,32,33,34,35]. Most of these reports regard Cyt-SOD; not so much information on EC-SOD is known in CNS. We previously reported that the expression level of ECSOD in cultured astrocytes was higher than neurons and microglia by semi-quantitative RT-PCR and that lipopolysaccharide-stimulation increased SOD activity in the medium [43]. We found that exposure to Ex-4 increased the EC-SOD expressions in mRNA and protein, and SOD activities on the cell-surface and in the medium were not significantly affected by Ex-4

Experimental procedures

Results

Discussion

Conclusion