Abstract
Abstract— We measured excision repair of ultraviolet radiation (UVR)‐induced pyrimidine dimers in DNA of the corneal epithelium of the marsupial, Monodelphis domestica, using damage‐specific nucleases from Micrococcus luteus in conjunction with agarose gel electrophoresis. We observed that 100 J ‐2 of UVR from aFS–40 sunlamp(280–400 nm) induced an average of 2.2 ± 0.2 times 10‐2 endonuclease‐sensitive sites per kilobase (ESS/kb) (pyrimidine dimers) and that ∼ 50% of the dimers were repaired within 12 h after exposure. We also determined that an exposure of 400 J m‐2 was needed to induce comparable numbers of pyrimidine dimers (2.5 times 10‐2) in the DNA of skin of M. domestica in vivo. In addition, we found that 50% of the dimers were also removed from the epidermal cells of M. domestica within 12 h after exposure. A dose of 100 J m‐2 was necessary to induce similar levels of pyrimidine dimers (2.0 ± 0.2 times 10‐2) in the DNA of the cultured marsupial cell line Pt K2 (Potorous tridactylus).
Published Version
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