Abstract
The excised root culture inoculation technique proved to be an accurate method for evaluating resistance to root-knot nematodes in tomato (Lycopersicon esculentum). Root cultures of seven tomato cultivars growing in an incubator at 25°C were inoculated with egg masses ofMeloidogyne javanica. Infection severity was determined 5 weeks after inoculation. Nematode-susceptible cultivars were characterized by high galling indices and resistant cultivars by low ones. The excised root culture technique is simple, allows results to be obtained rapidly, and no greenhouse space is needed.
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