Examination of Photo-, Mixo-, and Heterotrophic Cultivation Conditions on Haematococcus pluvialis Cyst Cell Germination

Andreas Bauer,Mirjana Minceva

doi:10.3390/app11167201

Abstract

The microalgae Haematococcus pluvialis is used for the biotechnological production of astaxanthin. The red carotenoid accumulates in the cytoplasm under unfavorable conditions. Astaxanthin synthesis is associated with the transformation of motile vegetative cells into non-motile cyst cells. In the industrial process, after harvesting, the cyst cells are mechanically disrupted, dried, and finally, astaxanthin is extracted with supercritical CO2. The germination of the cyst cells represents an interesting alternative, replacing the mechanical cyst cell wall disruption. When cyst cells are exposed to favorable growth conditions, germination of the cyst cells occurs and zoospores are released after a certain time. These zoospores show a much weaker cell matrix compared to cyst cells. In this study, germination under phototrophic, mixotrophic, and heterotrophic conditions was examined. Glucose was used as the carbon source for mixotrophic and heterotrophic germination. Applying heterotrophic conditions, up to 80% of the cells were in the zoospore stage 49 h after the start of germination, and extraction yields of up to 50% were achieved using the solvent ethyl acetate for the extraction of astaxanthin from the algal broth containing zoospores. An extraction yield of up to 64% could be achieved by doubling the nitrate concentration and combining mixotrophic and heterotrophic cultivation.

Highlights

The microalgae Haematococcus pluvialis (H. pluvialis) is the organism used for the biotechnological production of the red carotenoid astaxanthin [1]
The germination of the cyst cell of H. pluvialis represents an interesting alternative to conventional mechanical cell wall disruption [26]
It was found that more zoospores for both cultivation conditions and more astaxanthin could be extracted into ethyl acetate when gassed with ambient air compared to 3% v/v CO2

Summary

Introduction

The microalgae Haematococcus pluvialis (H. pluvialis) is the organism used for the biotechnological production of the red carotenoid astaxanthin [1]. H. pluvialis belongs to the Volvocales, which represent an order of flagellated green algae [4]. In nature, it is mainly found in temperate regions of the world, temporary water points, and natural or artificial freshwater pools [5]. The microalga live mainly as a green, flagellated vegetative cell (Figure 1a). The species undergoes asexual reproduction producing aplanospores (Figure 1b) which, when mature accumulate astaxanthin (Figure 1c) and become cyst cells (Figure 1d) and may produce zoospores, accumulating astaxanthin (Figure 1e)

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