Abstract
Excessive glutamate release following traumatic spinal cord injury (SCI) has been associated with exacerbating the extent of SCI. However, the mechanism behind sustained high levels of extracellular glutamate is unclear. Spinal cord segments mounted in a sucrose double gap recording chamber are an established model for traumatic spinal cord injury. We have developed a method to record, with micro-scale printed glutamate biosensors, glutamate release from ex vivo rat spinal cord segments following injury. This protocol would work equally well for similar glutamate biosensors.
Highlights
Protocol ArticleEx vivo electrochemical measurement of glutamate release during spinal cord injury James K
Subject Area: More specific subject area: Protocol name: Reagents/tools: Neuroscience Glutamate Excitotoxicity and Its Role in Spinal Cord Injury
Experimental design: Trial registration: Ethics: A spinal cord segment was isolated from a rat and maintained in oxygenated Krebs solution
Summary
Ex vivo electrochemical measurement of glutamate release during spinal cord injury James K. Tran N.H. Nguyena, Mara Fattaha, Jessica C. A Weldon School of Biomedical Engineering, Birck Nanotechnology Center, Center for Implantable Devices, Purdue University, West Lafayette, IN, USA b Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA
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