Abstract

In a series of bench scale activated sludge experiments, the evolution of sludge microbial communities was studied. The different communities required 2–3 months to reach functional optimum as measured by parameters such as substrate removal efficiency, effluent suspended solids and sludge volume index. Nevertheless, a period of at least 4 months, corresponding to 10 mean cell residence times, was necessary before full nitrification and minimum endogenous respiration were reached. Inoculation with wastewater sludge enhanced the evolvement of the microbial community, but was not essential. Activity parameters such as invertase and ATP-content, as well as the behaviour of the numerically dominant species, suggest that a microbial community evolves to a climax pattern rather than to a distinct type of climax state.

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