Abstract
Biologically active Low-molecular-weight, chromium-binding substance (LMCr) has been isolated from the liver of rabbits injected with potassium bicarbonate and cow's milk. This substance enhances glucose oxidation and lipogenesis from glucose in rat adipocytes [Yamamoto A. et al . (1987) Eur. J. Biochem. 165, 627–631; (1988) J. Nut. 118, 39–45]. LMCr was shown to lose its activity almost completely as an effectant of glucose metabolism when Cr was deleted under acidic conditions and separated from LMCr by EDTA-chelation and successive molecularsieve chromatography. Reincorporation of Cr 3+ into apo-LMCr resulted in 50–90% recovery of its original activity. These findings suggest that the biological activity of LMCr resides in Cr.
Published Version
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