Abstract
The aim of these experiments was to determine whether brown adipose tissue (BAT) is a glucocorticoid target organ. The interscapular fat pad from adrenalectomized rats was used in steroid-binding experiments to ascertain whether BAT contained glucocorticoid receptors. Bound and free steroids were separated by Sephadex chromatography. The time course of [3H]dexamethasone (9α-fluoro-16α-methyl- 16β,17α,21-trihydroxypregna-l,4-diene-3,20-dione) binding to BAT cytosol reached a plateau between 1 and 2 h at 0 C and remained stable for 5 h; there was extensive decay by 24 h. Cytosol incubated with increasing concentrations of [3H]dexamethasone for 3 h at 0 C exhibited a saturable number of binding sites. Scatchard analysis showed the binding to be to a homogeneous class of binding sites with an apparent dissociation constant of 6.5 nM and a concentration of 161 fmol/mg cytosol protein. The relative potency of a variety of steroids for the BAT-binding site was assessed by competi tive binding assay. The site res...
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