Abstract
Thermogenesis in brown adipose tissue (BAT) is controlled by sympathetic and sensory nerve fibres, and sympathetic innervation of white adipose tissue (WAT) is considered to control lipolysis and hormone production. Changes in innervation have been linked to altered physiological states but quantitative data on absolute values of BAT and WAT innervation and detailed data on neurochemical coding have not been provided yet. We here addressed these issues in interscapular (BAT) and ependidymal fat pad (WAT) of mice by single- and double-labelling immunofluorescence in wild-type and BAC(chrna3)-eGFP transgenic mice (sympathetic axons express eGFP), and innervation by sympathetic and CGRP-positive axons was assessed by stereology.
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