Evidence of cassava common mosaic virus in cassava shoot apical meristems

Abstract

The data that support the findings of this study are available from the corresponding author upon reasonable request. Figure S1 Effect of explant size on the regeneration of CsCMV-free plants. Each bar represents the mean ± SEM (n = 30). Different letters indicate statistically significant differences (one-way analysis of variance with Fisher’s LSD multiple comparison test, p ≤ 0.05). Figure S2 Electrophoresis of the reverse transcription-PCR products amplified with universal primers for Potexvirus, obtained from in vitro-generated cassava plants regenerated from explants of three sizes. (T1) shoot tip of 10 mm; (T2) shoot tip of 0.7 mm; (T3) shoot apical meristem of 0.2 mm; (C+) positive control; (C−) negative control; (CX) reaction control; (MWM) molecular weight marker 1 kb Plus DNA ladder. Figure S3 Electron micrographs of cassava plants infected with CsCMV. (a) Cells of the shoot tip (cross section) of cassava plants infected with CsCMV corresponding to T1. (b) and (c) Detail of CsCMV particles associated with the cell wall in T1. CW, cell wall; VA, vacuole; MI, mitochondrion; NU, nucleus; VP, viral particle. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.